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PfPLSCR is expressed in gametocytes. A) Immunofluorescence analysis of stage III-IV gametocytes reveals different localisation patterns of the triple-HA tagged protein. Anti-Pfs16 is used as gametocyte specific antibody marking the PVM. Parasite nuclei are stained with DAPI. Scale bar = 1 μM. B)PfPLSCR-HA co-localises with the IMC marker GAP45, which delineates the parasite periphery. C) Western blot analysis confirms PfPLSCR-HA expression in gametocytes.
