Table 1.

Ingredient composition and calculated and analyzed nutrient content of experimental diets¹ (as-fed basis)

Ingredient, %	CONT	DON1	DON3	DON5
Barley	44.0	44.0	44.0	44.0
Wheat	40.0	33.3	20.0	6.7
DON wheat2	0.0	4.9	14.8	24.7
Wheat screenings3	0.0	1.7	5.2	8.6
Soybean meal	10.0	10.0	10.0	10.0
Canola oil	3.5	3.5	3.5	3.5
l-Lysine–HCl	0.30	0.30	0.30	0.30
dl-Methionine	0.07	0.07	0.07	0.07
l-Threonine	0.10	0.10	0.10	0.10
Limestone	0.8	0.8	0.8	0.8
Dicalcium phosphate	0.5	0.5	0.5	0.5
Salt	0.5	0.5	0.5	0.5
Vitamin/mineral premix4	0.2	0.2	0.2	0.2
Calculated nutrient content5
ME, kcal/kg	3282	3282	3282	3282
Dry matter, %	86.49	86.51	86.54	86.58
Crude protein, %	15.9	15.9	15.9	16.0
Lysine, % SID6	0.76	0.76	0.76	0.76
Calcium, %	0.50	0.50	0.50	0.50
Phosphorus, %	0.48	0.48	0.48	0.48
Analyzed nutrient content
Growth performance diet
Dry matter, %	88.9	88.2	88.3	88.8
Crude protein, %	14.6	14.2	13.5	14.8
N-balance diet7
Dry matter, %	88.5	88.1	88.9	88.7
Crude protein, %	14.5	14.6	14.1	14.7

¹CONT, 0 DON; DON1, 1 ppm DON; DON3, 3 ppm DON; DON5, 5 ppm DON.

²DON wheat contains 6.9 ppm DON (Central Testing Laboratory, Winnipeg, MB, Canada).

³Wheat screenings contain 32.8 ppm DON (Central Testing Laboratory, Winnipeg MB, Canada).

⁴Supplied per kg of complete diet; vitamin A, 8,000 IU; vitamin D, 1,500 IU; vitamin E, 30 IU; menadione, 2.5 mg; vitamin B12, 0.025 mg; thiamine, 1.00 mg; biotin, 0.10 mg; niacin, 20 mg; riboflavin, 4 mg; pantothenate; 12 mg; folic acid, 0.50 mg; pyridoxine, 2.0 mg; Fe, 100 mg; Zn, 100 mg; Mg, 40 mg; Cu, 15 mg; Se, 0.30 mg; and I, 1 mg.

⁵Nutrient content of diets based on the nutrient content of feed ingredients according to NRC (2012) and analysis of nutrient content of wheat screenings (Central Laboratory Testing, Ltd, Winnipeg, MB, Canada).

⁶SID, standardized ileal digestible.

⁷Nitrogen balance diet contained 0.4% celite as a marker added at the expense of wheat.