Figure 3.

Temporal changes in marker expression during duct-like and acinus-like lineage specification

(A–D) RNA expression of pancreatic ductal markers SOX9 (A), HNF1B (B), CAII (C), and CFTR (D) in DUs and ACs during 16-day culture. Floating column charts represent RNA measurements from quantitative PCR, hinges represent maximal and minimal values, central lines indicate mean values, and dots represent individual measurements.

(E and F) Expression of SOX9 (E) and CAII (F) in organoids, detected by immunofluorescence. Numbers embedded in the images indicate the average percentage and standard deviation of marker-positive organoids. Scale bars, 50 μm.

(G–I) RNA expression of the pancreatic acinar markers PTF1A (G), RBPJL (H), and CPA1 (I) in DUs and ACs during 16-day culture. Floating column charts: representation as in (A).

(J and K) Expression of PTF1A (J) and CPA1 (K) in organoids, detected by immunofluorescence. Numbers embedded in images indicate the average percentage and standard deviation of marker-positive organoids. Scale bars, 50 μm.

All results represent the sum of three independent cultures.