Figure 6.

TGF-β augments KRAS^G12D-induced phenotypes in ACs and DUs

(A) Expression of the cell proliferation marker Ki67 (red) in DUs (blue) and ACs (orange) with KRAS^G12D expression and TGF-β treatment. Quantification of Ki67-positive organoids represented by box-and-whisker plots: range, 5%–95%; center lines indicate median values; gray dots represent individual measurement.

(B and C) Organoid morphological changes induced by KRAS^G12D expression and TGF-β treatment. Bar charts represent the mean and standard deviation of measurements from three independent cultures; gray dots represent individual measurements.

(D) Organoid size changes induced by KRAS^G12D expression and TGF-β treatment. Box-and-whisker plot representation as in (A).

(E and F) Expression of pancreatic lineage markers in DUs (E) and ACs (F) altered by KRAS^G12D expression and TGF-β treatment. Floating column charts represent RNA measurements from quantitative PCR (n = 3), hinges represent maximal and minimal values, central lines indicate mean values, and dots represent individual measurements.

(G–J) Detection of KRT7 (G), AB (H), MUC2 (I), and MUC5AC (J) in organoids under different experimental conditions. Bar chart representation as in (C).

Scale bars in main images, 50 μm; scale bars in insets, 25 μm. ^∗∗∗p < 0.001; ^∗∗, 0.001 < p < 0.01; ^∗, 0. 01 < p < 0.05; N.S., p > 0.05. All results represent the sum of three independent cultures.