REAGENTS or RESOURCE	SOURCE	IDENTIFIER
Antibodies
SOX9	Millipore	AB5535
CAII	Santa Cruz	sc-133111
PTF1A	Christopher Wright Lab (Vanderbilt)	N/A
PTF1A	BD Biosciences	s25-763
CTRC	Sino Biological	11456-T24
CPA1	Sino Biological	10504-RP02
MUC2	Santa Cruz	sc7314
MUC5AC	Santa Cruz	sc33667
phospho-ERK	Cell Signaling	4370
KRAS	Cell Signaling	3339
ERK	Cell Signaling	9107
KRT19	Abcam	ab9221
Ki67	Cell Signaling	9027
KRT7	DAKO	M7018
P16	Santa Cruz	sc-1661
Goat anti-mouse IgG	Invitrogen	A-21424
Goat anti-rabbit IgG	Invitrogen	A-11034
Oligonucleotides
TBP (PCR primers)	IDT	Hs.PT.39a.22214825
PDX1 (PCR primers)	IDT	Hs.PT.58.2295117
NXK6-1 (PCR primers)	IDT	Hs.PT.58.25073618
GATA4 (PCR primers)	IDT	Hs.PT.58.259457
SOX9 (PCR primers)	IDT	Hs.PT.58.38984663
CFTR (PCR primers)	IDT	Hs.PT.58.3365414
PTF1A (PCR primers)	IDT	Hs.PT.58.45382933.g
CPA1 (PCR primers)	IDT	Hs.PT.58.3138313
CTRC (PCR primers)	IDT	Hs.PT.58.15671909
HNF1B (PCR primers)	IDT	Hs.PT.58.25568705
RBBPJL (PCR primers)	IDT	Hs.PT.58.15167632.gs
Critical commercial assays
Amylase Activity Colorimetric Assay Kit	Biovision	K711
Lipase Activity Fluorometric Assay Kit III	Biovision	K724
Anhydrase (CA) Activity Assay Kit	Biovision	K472
Alcian Blue (pH 2.5) Stain Kit	Vector Laboratories	H3501
Deposited data
Single nuclei transcriptome	GEO repository	GEO: GSE169008
Experimental models: cell lines
HUES8	Douglas Metlon Lab (Harvard University)	CVCL_B207
iPSC #11	Douglas Metlon Lab (Harvard University)	N/A
iPSC #13	Douglas Metlon Lab (Harvard University)	N/A
Experimental models: organisms
NCG mice	Charles River	572
Recombinant DNA
pINDUCER21 (ORF-EG)	Addgene	#46948
Biological samples
PDAC patient tissue #1 (paraffin slide)	BIDMC	N/A
PDAC patient tissue #2 (paraffin slide)	BIDMC	N/A
Software and algorithms
Analysis Code	Github	https://github.com/dannyconrad/PancreasOrganoidMULTIseq
Chemicals, peptides, and recombinant proteins
Forskolin	Selleckchem	S2449
BSA (fatty acid-free)	Proliant Biologicals	68700
PBS	Invitrogen	14155063
DEMEM	Invitrogen	10564045
TrypLE	Invitrogen	12605010
Matrigel	Corning	354263
Cell Recovery Solution	Corning	354253
Trizol	Invitrogen	15596018
Direct-zol	Zymo Research	R2080
Superscript IV	Invitrogen	11756500
STEMxyme I	Worthington Biochemical	STZ1
Doxycycline diet	Envigo	TD01306
TGF	Peprotech	100-21
FGF10	Peprotech	100-26
FGF2	Peprotech	100-18C
EGF	Peprotech	AF-100-15
Doxycycline (powder)	Tocris	4090
A83-01	Tocris	2939
Y27632	Tocris	1245
SKL2001	Selleckchem	S8320
WNT1	Peprotech	120-17
Foxy5	Tocris	5461
Dexamethasone	Tocris	1126
LDN193189	Tocris	6053
CD3254	Tocris	3302
BMS961	Tocris	3410
DBZ	Tocris	4489
HPI-1	Tocris	3839
XMU-MP-1	Selleckchem	S8334
CHIR99021	Tocris	4953
Ascorbic acid	Sigma	A4544
EPZ011989	Selleckchem	S7805
IWP2	Tocris	3533
IQ1	Tocris	4713
iCRT-14	Tocris	4299
CPTH2	Cayman Chemical	12086
SB939	Selleckchem	S1515
WT161	Selleckchem	S8495
XAV939	Tocris	3748
FGF1	Peprotech	100-17A