Figure 8.

Obacunone suppressed mRNA and protein levels of cell cycle-associated genes in vitro. Flow cytometry was used to analyze the cell cycle of Caco2 cells treated with or without obacunone (A). Relative cell numbers in the G1 and G2 phases (B). Levels of proteins related to the cell cycle in Caco2 cells were determined via WB and their relative densities calculated (C). Relative levels of mRNAs associated with the cell cycle in Caco2 cells treated with/without obacunone were assessed via qPCR (D). Values are presented as means ± SD (n = 3). vs control group, *p < 0.05, **p < 0.01, ***p < 0.001.