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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Comp Neurol. 2020 Dec 17;529(9):2176–2188. doi: 10.1002/cne.25084

FIGURE 2.

FIGURE 2

Differences in Syt2a and Syn1/2 expression during development. (a) Sections showing Syt2a and Syn1/2 expression in the brains of 6, 11, 14, and 18 dpf zebrafish larvae show that the Syn1/2 expression (magenta) throughout the forebrain (fb) appears not to change. In contrast, Syt2 expression (green) becomes more prominent over time selectively in the forebrain. Level of Syt2a expression in the optic tectum (OT) remains similar among different time points. Medial section (see cartoon in (d)). Asterisks mark the anterior forebrain. (b) Higher magnification of the forebrain region reveals robust and strong Syt2a expression in the olfactory bulb (olf), its projections (olf-p) and in the ventral telencephalon (vT) at 14 dpf, which broadens and becomes more visible in the dorsal telencephalon (dT) at 18 dpf. Arrows point to Syt2a puncta within these regions. (c) Lateral sections (see (d)) reveal an increase in Syt2a signal in the olf, dT, and the dorsal and lateral area of the anterior commissure (AC) between 6 and 14 dpf. (d) Cartoon showing a dorsal view of a zebrafish brain marking the level of the lateral and medial sections shown in the images. Scale bars are 100 μm in (a), 50 μm in (b), and 30 μm in (c)