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CAFs secreted exosomal miR-103a-3p in NSCLC. (A) Immunofluorescence staining and (B) Western blot for α-SMA, FAP, and FSP1 expression of NFs and CAFs. (C) qRT-PCR analyzed the expression of miR-103a-3p in TCs, NFs and CAFs. (D) TEM of exosomes isolated from TCs, NFs and CAFs. (E) The expression of CD63, Alix, and Tsg101 in exosomes was detected by western blot. (F) The expression of miR-103a-3p in exosomes form TCs, NFs and CAFs was tested by qRT-PCR. *p<0.05.
