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. 2021 May 18;13(10):14258–14276. doi: 10.18632/aging.203042

Figure 2.

Figure 2

LncRNA LOC100129620 overexpression promotes the proliferation and invasion of osteosarcoma cells. (A) The expression of LOC100129620 in U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (B) CCK-8 assay to analyze the viability of U2OS cells transfected with pLVX-Vector or pLVX-LOC100129620. (C) CCK-8 assay to analyze the viability of MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (D) Colony formation assay to analyze the proliferation of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (E) Quantitative analysis of colony formation of U2OS and MG63 cells with transfected pLVX-Vector or pLVX-LOC100129620. (F) Flow cytometry analysis of the cell cycle in U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (G) Flow cytometry analysis of apoptosis of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (H) Cell scratch assay to detect the migration ability of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (I) Transwell assay to detect the invasion ability of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. (J) Quantitative analysis of the Transwell assay results of U2OS and MG63 cells transfected with pLVX-Vector or pLVX-LOC100129620. Statistical analysis was conducted using Student’s t-test. Values are means ± SD. *P < 0.05 and **P < 0.01.