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. 2021 May 18;13(10):14258–14276. doi: 10.18632/aging.203042

Figure 3.

Figure 3

LncRNA LOC100129620 knockdown inhibits the proliferation and invasion of osteosarcoma cells. (A) The expression of LOC100129620 in U2OS cells transfected with pLKO.1-Vector, pLKO.1-sh1, or pLKO.1-sh2. (B) The expression of LOC100129620 in MG63 and MG63 cells transfected with pLKO.1-Vector, pLKO.1-sh1, or pLKO.1-sh2. (C) CCK-8 assay to analyze the viability of U2OS cells transfected with pLKO.1-Vector, pLKO.1-sh1, or pLKO.1-sh2. (D) CCK-8 assay to analyze the viability of MG63 cells transfected with pLKO.1-Vector, pLKO.1-sh1, or pLKO.1-sh2. (E) Colony formation assay to analyze the proliferation of U2OS and MG63 cells transfected with pLKO.1-Vector, pLKO.1-sh1, or pLKO.1-sh2. (F) Quantitative analysis of the colony formation assay results of U2OS and MG63 cells transfected with pLKO.1-Vector, pLKO.1-sh1, or pLKO.1-sh2. (G) Flow cytometry analysis of the cell cycle in U2OS and MG63 cells transfected with pLKO.1-Vector, pLKO.1-sh1, or pLKO.1-sh2. (H) Flow cytometry analysis of apoptosis of U2OS and MG63 cells transfected with pLKO.1-Vector, pLKO.1-sh1, or pLKO.1-sh2. (I) Cell scratch assay to detect the migration ability of U2OS and MG63 cells transfected with pLKO.1-Vector, pLKO.1-sh1, or pLKO.1-sh2. (J) Transwell assay to detect the invasion ability of U2OS and MG63 cells transfected with pLKO.1-Vector, pLKO.1-sh1, or pLKO.1-sh2. (K) Quantitative analysis of the Transwell assay results of U2OS and MG63 cells transfected with pLKO.1-Vector, pLKO.1-sh1, or pLKO.1-sh2. Statistical analysis was conducted using Student’s t-test. Values are means ± SD. *P < 0.05 and **P < 0.01.