Table 1. Primer sequences for RT-qPCR.
Gene | Primer sequence (5'-3') |
miR-1246 | F: TGAAGTAGGACTGGGCAGAGA |
R: TGTTTGCAATAGCCCTTTGAG | |
U6 | F: CTCGCTTCGGCAGCACATA |
R: GTGCAGGGTCCGAGCT | |
LRIG1 | F: GGTGAGCCTGGCCTTATGTGAATA |
R: CACCACCATCCTGCACCTCC | |
GAPDH | F: TGCACCACCAACTGCTTAGC |
R: GGCATGGACTGTGGTCATGAG |
Note: RT-qPCR, reverse transcription-quantitative polymerase chain reaction; F, forward; R, reverse; miR-1246, microRNA-1246; LRIG1, leucine rich repeats and immunoglobulin like domains 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.