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. 2021 May 18;13(10):14198–14218. doi: 10.18632/aging.203037

Figure 1.

Figure 1

LOC146880 expression is upregulated in ESCC tissues and cells. (A) The expression levels of LOC146880 in 21 pairs of ESCC and adjacent normal esophageal tissues. (B) Kaplan-Meier survival curve analysis shows overall survival of ESCC patients with high- and low-expression levels of LOC146880 (n = 81). (C) QRT-PCR analysis shows LOC146880 expression levels in human esophageal epithelial cells and ESCC cell lines (EC109, TE-1, Kyse30, Kyse70, Kyse150, and Kyse410). (D) FISH analysis shows LOC146880 expression in ESCC and normal esophageal tissues. (E) Bioinformatics analysis (https://genome.ucsc.edu/) shows H3K27ac levels in the promoter region of LOC146880. (F) ChIP assay analysis with anti- H3K27ac antibody shows H3K27ac levels in the LOC146880 promoter of HEEC, Kyse30, and TE-1 cells. (G) ChIP assay analysis with anti-CBP antibody shows CBP protein levels in the LOC146880 promoter of HEEC, Kyse30, and TE-1 cells. (H) Western blot analysis shows CBP protein levels in control and CBP-silenced Kyse30, and TE-1 cells. (I) ChIP assay analysis shows H3K27ac levels in the LOC146880 promoter of control and CBP-silenced Kyse30, and TE-1 cells. (J) QRT-PCR analysis shows LOC146880 expression levels in control and CBP-silenced Kyse30 and TE-1 cells. *P < 0.05, **P < 0.01, ***P < 0.001.