Table 9. Sequence of primers used in RT-qPCR.
Primer | Primer sequence (5'-3') | |
GAPDH | F: CTGACCATGCCGCCTGGAGA | R: ATGTAGGCCATGAGGTCCAC |
U6 | F: ATGACGTCTGCCTTGGAGAAC | R: TCAGTGTGCTACGGAGTTCAG |
Sirt1 | F: GTCTGTGCCTTCCAGTTGCT | R: CTGCTTGCTGTCCATACCTG |
NF-κBp65 | F: GTGCAGAAAGAAGACATTGAGGTG | R: AGGCTAGGGTCAGCGTATGG |
MiR-155 | F: GGAGGTTAATGCTAATTGTGATAG | R: GTGCAGGGYCCGAGG |
BNDF | F: CTGCTTCAGTTGGCCTTTCG | R: TGCTGTGGTGGTGATTGCCTCTGTG |
Note: F, forward; R, reverse; GAPDH, glyceraldehyde phosphate dehydrogenase; miR-155, microRNA-155; BNDF, brain-derived neurotrophic factor.