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. 2021 May 31;8:659840. doi: 10.3389/fvets.2021.659840

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Copyright © 2021 Yan, Zhang, Gao, Zhang, Zhang, Chen, Lin and Xie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the hypomethylated gene TGFB2 in ALV-J- positive chicken samples vs. ALV-J- negative chicken samples by bisulfite sequencing. (A) Visualization of the DMRs in TGFB2, using the IGV tool. DMR is indicated by boxes above the tracks. Scales are showed on the left; (B) the validation result of the DMRs of TGFB2 in the promoter region by bisulfate sequencing. Black circles indicate the methylated CpG locus and white circles indicate the unmethylated CpG locus. The chi-square test showed the differences between ALV-J-negative and ALV-J-positive chickens.