Table 4.
Effects of the drugs at MIC on the expression of the genes significantly (P ≤ 0.05) after 4 h and 16 h incubation (fold-change downregulation and P values, *: fold-change upregulation).
| Drugs and incubation time | Genes of MRSA#1 | Genes of MRSA#2 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| spa | sarA | RNAIII | sigB | mepA | mgrA | srtA | spa | sarA | RNAIII | sigB | mepA | mgrA | srtA | ||
| 4 h | ASA | 15(˂0.001) | - | - | 5.6(˂0.001) | 6.5(0.028) | 4.7(0.027) | 6.6(0.001) | 17.9(0.002) | 6.4(0.036) | - | 5.9(0.027) | - | - | 8.4(0.023) |
| ACE | 27.7(˂0.001) | - | - | 5.8(0.001) | - | 4.6(0.034) | 3.4(0.009) | 21.4(0.002) | 9.9(0.024) | - | 11.8(0.016) | - | - | 8.8(0.022) | |
| DIC | 7.2(˂0.001) | - | - | 2.2(0.003) | - | - | 2.2(0.012) | 2.1(0.015) | - | - | -/3.1(0.006) | - | -/3.7(0.014) | - | |
| IBU | 28.9(˂0.001) | - | - | 9(˂0.001) | 12.3(0.022) | 4.6(0.029) | 9.1(0.001) | 26.5(0.002) | 5.1(0.031) | - | 9/8.3(0.017/0.007) | -/12.7(0.012) | -/8.4(0.007) | 20.3(0.017) | |
| NAP | 23.8(˂0.001) | - | - | 5(˂0.001) | 6.2(0.031) | - | 7.1(0.001) | 6.1(0.006) | - | - | - | - | - | 10.8(0.02) | |
| 16 h | ASA | - | 5.4(0.007) | 5.7(0.04) | 7.1(0.025) | 6.1/4.8(˂0.001/0.034) | 6.6(0.034) | 6.7(0.001) | 8(0.004) | - | - | 10.1(0.027) | 16(0.039) | 15.4(0.01) | 13.8(0.004) |
| ACE | - | 11.8(0.004) | 8.3(0.033) | 12.9(0.02) | 13(˂0.001) | 13.3(0.026) | 14.7(0.001) | 12.7(0.003) | - | - | 30.4(0.021) | 25.9(0.036) | 31.4(0.009) | 24.3(0.003) | |
| DIC | - | - | - | - | - | - | 1.7(0.026) | 3.14(0.016) | - | - | 8.9(0.029) | 8.4(0.048) | 10.3(0.012) | 6.4(0.007) | |
| IBU | - | - | - | 9(0.022) | 9.2(˂0.001) | 4.6(0.043) | 7.2(0.001) | - | - | - | - | - | - | - | |
| NAP | - | - | - | - | 2.5/5.9(0.003/0.031) | - | 3.2(0.004) | 3.2(0.013) | - | - | 10.1(0.027) | - | 13.3(0.011) | 10.1(0.005) | |
| Drugs and incubation time | Genes of MRSA#3 | Genes of ATCC strain | |||||||||||||
| spa | sarA | RNAIII | sigB | mepA | mgrA | srtA | spa | sarA | RNAIII | sigB | mepA | mgrA | srtA | ||
| 4 h | ASA | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
| ACE | - | - | - | 6(0.015) | 3.7(0.005) | 4.4(0.009) | 4(0.027) | - | - | - | - | - | - | - | |
| DIC | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
| IBU | - | - | - | 6.3(0.013) | 3.2(0.007) | 6.2(0.005) | 6.4(0.015) | - | - | - | - | - | - | - | |
| NAP | - | - | - | 11.8(0.01) | 3.8(0.005) | 5(0.006) | 11.3(0.011) | - | - | - | - | - | - | - | |
| 16 h | ASA | - | - | - | - | - | 1.9(0.01) | - | - | - | - | 2.7(0.018) | 3.7(0.004) | 3.7(0.007) | - |
| ACE | - | - | 2.8(0.024) | - | - | 2.6(0.003) | - | - | - | - | 2.8(0.021) | 6.4(˂0.001) | 3.7(0.011) | 2.9(0.042) | |
| DIC | - | -/*1.8(0.032) | - | -/*1.6(0.044) | -/*2.4(0.042) | -/*2.7(0.04) | - | - | - | - | - | - | - | - | |
| IBU | - | - | - | - | - | - | - | *5.4(0.047) | - | - | *23.1(0.031) | - | *24.4(0.03) | *27(0.032) | |
| NAP | - | - | - | - | - | - | - | - | - | - | - | 2(0.009) | - | - | |
MIC: minimum inhibitory concentration, (-): no alteration, (bold): alteration with 100 µg/mL drugs, the data was the mean of three replicates.