Figure 3.

(A) Bacterial burden by qPCR endpoint fluorescence in basilar parenchyma tissue across all diseases and CORE lungs. Significant differences in bacterial burden were identified between IPF and CTD-ILD, IPF and CF, and IPF and CORE basilar tissue. (B) Alpha diversity, as measured by Shannon index, of basilar parenchyma tissue across all diseases and CORE lungs. IPF basilar tissue had significantly higher alpha diversity compared to CF basilar tissue. (C) Beta diversity, as measured by Bray-Curtis dissimilarity in basilar parenchyma tissue of IPF, CF, and CORE lungs, demonstrating significant taxonomic composition differences by disease. P-values are obtained by Wilcoxon tests. *= p<0.05, ***=p<0.001, ****=p<0.0001. IPF, idiopathic pulmonary fibrosis; CTD-ILD, connective tissue disease-associated interstitial lung disease; COPD, chronic obstructive pulmonary disease; CF, cystic fibrosis; CORE, Center for Organ Recovery and Education; MDS1, primary NMDS axis; MDS2, secondary NMDS axis; NMDS, non-metric multidimensional scaling.