Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | BL21(DE3) | Sigma-Aldrich | CMC0016 | Chemically competent cells |
| Recombinant DNA reagent | pMAL c2x DWORF plasmid | This study | MBP-DWORF fusion; H.S. Young lab | |
| Peptide, recombinant protein | Human DWORF | This study | NCBI: NP_001339058.1 |
Purified human DWORF peptide; H.S. Young lab |
| Chemical compound, drug | CDN1163; 4-(1-methylethoxy)-N-(2-methyl-8-quinolinyl)-benzamide | Sigma-Aldrich | SML1682 | Previously published SERCA activator (PMID:26702054) |
| Chemical compound, drug | C12E8; octaethylene glycol monododecyl ether | Nikkol | BL-8SY | Detergent for solubilizing SERCA |
| Chemical compound, drug | Egg PC; L-α-phosphatidylcholine (Egg, Chicken) | Avanti | 840051 | Natural lipid |
| Chemical compound, drug | Egg PE; L-α-phosphatidylethanolamine (Egg, Chicken) | Avanti | 840021 | Natural lipid |
| Chemical compound, drug | Egg PA; L-α-phosphatidic acid (Egg, Chicken) | Avanti | 840101 | Natural lipid |
| Chemical compound, drug | Ionomycin, calcium salt | Sigma-Aldrich | I3909 | Facilitates the transport of calcium across the plasma membrane |
| Chemical compound, drug | Ruthenium red | Sigma-Aldrich | R2751 | RyR inhibitor |
| Chemical compound, drug | Caffeine | Sigma-Aldrich | C0750 | RyR2 agonist |
| Recombinant DNA reagent | pEGFP_RyR2 | Donated by Dr. C. George (PMID:15047862) | Mammalian expression construct containing GFP fused RyR2 | |
| Cell line (HEK 293) | T-Rex-293 Cell Line | Thermo Fisher Scientific |
R71007 | Mammalian cell line for the stable expression of proteins of interest |
| Recombinant DNA reagent | EYFP-PLN | Singh et al., 2019 | Constitutively fluorescent green/yellow fluorescent protein fusion construct | |
| Recombinant DNA reagent | EYFP-DWORF | Singh et al., 2019 | Constitutively fluorescent green/yellow fluorescent protein fusion construct | |
| Recombinant DNA reagent | mCerulean-SERCA | Bovo et al., 2019 | Fluorescent protein fusion construct | |
| Cell line (HEK 293) |
AAV-293 cells | Agilent Technologies | 240073 | HEK293 cell line optimized for AAV transfection |