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. 2021 Jun 9;10:e58688. doi: 10.7554/eLife.58688

Figure 3. Effect of LOX inhibition on ECM architecture in EGI-1, KPC, MMTV-PyMT, and mPDAC tumor models.

(A) Collagen fiber width distribution measured from SHG images (***p-value<0.001, Student’s t-test, 50–70 images/tumor, n = 3 mice/group from three independent experiments). (B) Fiber orientation defined by the coefficient of variation (CV) (***p-value<0.001, Student’s t-test, 40–50 images/tumor, n = 3 mice/group from three independent experiments). (C) Representative example of fiber orientation distribution. (D) Collagen fiber curvature defined by the curvature ratio (***p-value<0.001, Student’s t-test, 40–50 images/tumor, n = 3 mice/group from three independent experiments). (E) Percentage of red-orange birefringent fibers combining Red Sirius staining and polarized microscopy, orange-red fibers correspond to thick and packed regions (*p-value<0.05, ***p-value<0.001, Student’s t-test, 20–30 images/tumor, n = 3 mice/group from three independent experiments). (F) SHG images of collagen networks in EGI-1, MMTV-PyMT, mPDAC, and KPC control and BAPN-treated tumors. Illustrative scheme indicating the changes in width, orientation and curvature of collagen fibers induced by BAPN treatment. Scale bar = 50 µm. Images are representative of three experiments.

Figure 3—source data 1. Source data file for Figure 3.

Figure 3.

Figure 3—figure supplement 1. Combination of Sirius Red staining and polarized microscopy.

Figure 3—figure supplement 1.

Surface covered by red-orange birefringent fibers that correspond to thick and packed regions in EGI-1 MMTV-PyMT, mPDAC, and KPC tumor models. Scale bar = 100 µm.