| Genetic reagent (Mus musculus) |
Ptf1a
CreERT
|
Jackson Lab |
No: 019378 |
|
| Genetic reagent (M. musculus) |
Lox-Stop-Lox-Kras
G12D
|
Jackson Lab |
No: 008179 |
|
| Genetic reagent (M. musculus) |
Arid1aflox |
PMID:18448678
|
|
Dr. Zhong Wang lab (University of Michigan) |
| Cell line (Homo sapiens) |
hTERT-HPNE |
Dr. Jennifer Baily lab (UT Health) |
RRID:CVCL_C466
|
|
| Recombinant DNA reagent |
pInducer20-KRASG12D
|
Dr. Haoqiang Ying lab (MD Anderson Cancer Center) |
|
Inducible expression of KRASG12D
|
| Recombinant DNA reagent |
pL-CRISPR.EFS.tRFP |
Addgene |
RRID:Addgene_57819
|
Pol III-based sgRNA expression backbone |
| Recombinant DNA reagent |
pGIPZ |
Open Biosystems |
|
Pol III-based shRNA expression backbone |
| Sequence-based reagent |
sgARID1A |
This paper |
sgRNA |
CAGCGGTACCCGATGACCAT |
| Sequence-based reagent |
shALDH1A1 |
This paper |
shRNA |
GGAGTGTTTACCAAAGACATT |
| Sequence-based reagent |
shEP300-1 |
This paper |
shRNA |
CGGCAAACAGTTGTGCACA |
| Sequence-based reagent |
shEP300-2 |
This paper |
shRNA |
AGCTACTGAAGATAGATTA |
| Sequence-based reagent |
shNR3C1-1 |
This paper |
shRNA |
CCAACGGTGGCAATGTGAA |
| Sequence-based reagent |
shNR3C1-2 |
This paper |
shRNA |
AGCTGTAAAGTTTTCTTCA |
| Commercial assay or kit |
ROS detection assay kit |
BioVision |
Cat# K936-250 |
|
| Commercial assay or kit |
Senescence β-Galactosidase Staining Kit |
Cell Signaling Technology |
Cat# 9860 |
|
| Chemical compound, drug |
ALDEFLUOR diethylaminobenzaldehyde (DEAB) reagent, 1.5 mM in 95% ethanol |
Stemcell Technologies Inc |
Cat# 01705 |
|
| Antibody |
(Rabbit polyclonal) anti-ALDH1A1 |
Abcam |
Abcam Cat# ab23375, RRID:AB_2224009
|
WB(1:400) |
| Antibody |
(Rabbit polyclonal) anti-Aldh3a1 |
Abcam |
Abcam Cat# ab76976, RRID:AB_1523110
|
IHC(1:100) |
| Antibody |
(Rabbit polyclonal) anti-Ras |
Abcam |
Abcam Cat# ab180772, RRID:AB_2884935
|
WB(1:500) |
| Antibody |
(Mouse monoclonal) anti-β-Actin |
Sigma-Aldrich |
Sigma-Aldrich Cat# A1978, RRID:AB_476692
|
WB(1:4000) |
| Antibody |
(Rabbit monoclonal) anti- Phospho-p44/42 MAPK (Erk1/2) |
Cell Signaling Technology |
Cell Signaling Technology Cat# 4370, RRID:AB_2315112
|
WB(1:1000) |
