Figure 5.

miR-210-3p facilitates A549 cell viability, migration, and invasion but induces apoptosis by inhibiting PCGF3. (A) Two pieces of sh-PCGF3 were designed and independently delivered into A549 cells to construct USF1 knockdown A549 cells. (B) Real-time qPCR examined the expression of miR-210-3p in A549 cells treated with miR-210-3p inhibitor alone or with sh-PCGF3. (C) Immunoblots and quantification of USF1 and PCGF3 in A549 cells treated with miR-210-3p inhibitor alone or with sh-PCGF3. (D) Viability of A549 cells treated with miR-210-3p inhibitor alone or with sh-PCGF3 was examined by CCK-8 assays. (E) Apoptosis of A549 cells treated with miR-210-3p inhibitor alone or with sh-PCGF3 was analyzed by flow cytometry. (F) Representative view (×200) of A549 cells treated with miR-210-3p inhibitor alone or with sh-PCGF3 migrating from upper into lower chambers and statistics of migrating cells; Representative view (× 200) of cells invading from Matrigel-coated chambers into lower wells and statistics of invading cells. (G) Immunoblots and quantification of Bax, Bcl-2, MMP-2, and MMP-9 in A549 cells treated with miR-210-3p inhibitor alone or with sh-PCGF3. *p < 0.05 compared to sh-NC or NC inhibitor + sh-NC and ^#p < 0.05 compared to miR-210-3p inhibitor + sh-NC by ANOVA adjusted by Tukey’s test or by repeated measurements ANOVA adjusted by Bonferroni test (only for D).