TABLE 1.

Primer sequence of aging-related genes for qPCR.

Gene	Primer
AREG	Forward	TGT​CGC​TCT​TGA​TAC​TCG​GC
	Reverse	AGG​CAT​TTC​ACT​CAC​AGG​GG
ATG3	Forward	GTG​TTC​AGT​TCA​CCC​ATG​CAG
	Reverse	TTA​ACA​GCC​ATT​TTG​CCA​CTA​ATC​T
E2F1	Forward	CAT​CCC​AGG​AGG​TCA​CTT​CTG
	Reverse	GAC​AAC​AGC​GGT​TCT​TGC​TC
FOXO3	Forward	CGG​ACA​AAC​GGC​TCA​CTC​T
	Reverse	GGA​CCC​GCA​TGA​ATC​GAC​TAT
HDAC1	Forward	TTTTTGGGTYGGAYGTTGAG
	Reverse	CCCTCRCAACCTCCTCTCC
MMP2	Forward	TGG​CAC​CCA​TTT​ACA​CCT​AC
	Reverse	CCT​CGT​ATA​CCG​CAT​CAA​TC
NUF2	Forward	TGT​TAA​GCA​ATA​CAA​ACG​CAC​AG
	Reverse	TGC​CTT​TTC​AAT​ACC​GTC​GTG
TGFB1	Forward	CGA​CTC​GCC​AGA​GTG​GTT​AT
	Reverse	GCT​AAG​GCG​AAA​GCC​CTC​AA
TP53	Forward	AAG​TCT​GTG​ACT​TGC​ACG​TAC​TCC
	Reverse	GTC​ATG​TGC​TGT​GAC​TGC​TTG​TAG
β-actin	Forward	TTC​CAG​CCT​TCC​TTC​CTG​GG
	Reverse	TTG​CGC​TCA​GGA​GGA​GCA​AT