Fig. 1.

Identification of linear OspA epitopes. A series of OspA subfragments were generated with N-terminal S-tag fusions and purified. The recombinant proteins were separated by SDS-PAGE, transferred to membranes and screened with α-His tag Ab (top panel) or serum harvested from individual mice (M1 through M5) that were infected through needle inoculation with B. burgdorferi B31MI. Detection of Ab binding was performed as detailed in the text. The portion of OspA represented in each fragment is indicated above each lane.