Table 1.

Test methods used in the multi-pathogen molecular assay for respiratory pathogens

	Pathogen	Test method
Virus	Measles virus	Real-time RT-PCR
	Mumps virus	Real-time RT-PCR
	Rubella virus	Real-time RT-PCR
	Varicella zoster virus	Real-time RT-PCR
	Hantaan virus	Real-time RT-PCR
	Influenza A and B viruses	Real-time RT-PCR
	SARS coronavirus	Real-time RT-PCR
	Animal influenza virus (H5N1, H7N9)	Real-time RT-PCR
	MERS coronavirus	Real-time RT-PCR
	Adenovirus	Real-time PCR
	Human bocavirus	Real-time PCR
	Parainfluenza virus	Real-time RT-PCR
	Respiratory syncytial virus	Real-time RT-PCR
	Human rhinovirus	Real-time RT-PCR
	Human metapneumovirus	Real-time RT-PCR
	Human coronavirus	Real-time RT-PCR
	Enterovirus*	Real-time RT-PCR
Bacteria	Corynebacterium diphtheriae	PCR
	Bordetella pertussis	Real-time PCR
	Haemophilus influenzae type b	Real-time PCR
	Streptococcus pneumoniae	Real-time PCR
	Bacillus anthracis	Real-time PCR
	Mycobacterium tuberculosis	Real-time PCR
	Neisseria meningitidis	Real-time PCR
	Group A β-hemolytic streptococci	Real-time PCR
	Legionella spp.	Real-time PCR
	Yersinia pestis	Real-time PCR
	Francisella tularensis	Real-time PCR
	Coxiella burnetii	Real-time PCR
	Mycoplasma pneumoniae	Real-time PCR
	Chlamydia pneumoniae	Real-time PCR
	Chlamydia psittaci*	Real-time PCR
Fungi	Coccidioides immitis	PCR

All test methods were performed according to SOPs of the KDCA.

^*The molecular assays for enterovirus and Chlamydia psittaci were added following the advice of the board members of Korean Society for Laboratory Medicine (KSLM).

Abbreviations: SOPs, standard operating procedures; SARS, severe acute respiratory syndrome; MERS, Middle East respiratory syndrome.