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. 2021 Jun 15;131(12):e139202. doi: 10.1172/JCI139202

Figure 5. HIF-dependent expression of ANGPTL4 and VEGF in retinal organoids.

Figure 5

(A and B) D120 hiPSC-derived retinal organoids were treated with hypoxia (1–5 days) in the presence or absence of digoxin (30 μM) and the expression of VEGF and ANGPTL4 mRNA was examined by qPCR. (C) Müller cells isolated from retinal organoids expressed key Müller cell markers (vimentin and CRALBP). (D) Accumulation of HIF-1α and HIF-2α protein in hiPSC-derived Müller cells treated with hypoxia (12 hours) was blocked by digoxin (at indicated doses). (E and F) Expression of VEGF (E) and ANGPTL4 (F) mRNA in hiPSC-derived Müller cells treated with hypoxia (12 hours) inhibited by digoxin (3 μM). One-way ANOVA with Bonferroni’s multiple-comparison test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.