Figure 1. Characterization of CSF EV concentration and size using NTA.
(A) A subset of CSF samples (n = 5 HD, 5 control) was used to determine the size distribution of EVs using F-NTA. CSF EV particle concentration was characterized by EV size subgroups in increments of 25 nm (2-way ANOVA, Bonferroni’s multiple comparisons test, P > 0.05), as well as by (B) mode size (Student’s t test, unpaired, 2-tailed, P > 0.05), and (C) concentration (Student’s t test, unpaired, 2-tailed, P > 0.05). Data are representative of 1 independent experiment performed in triplicate with 5 HD and 5 control samples. Error bars depict mean ± SEM.