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. 2020 Oct 22;28(6):649–666. doi: 10.1038/s41417-020-00246-9

Fig. 5. Effect of Gi, Gq and MMPs inhibitors on vp17s-induced B-cell clonogenic activity.

Fig. 5

A, C, E Raji cells were cultured for 8 days in the presence or absence of EGF (100 ng/ml) or NHL-a101 or NHL-a102 (10 ng/ml) and pertussis toxin (PTX; 10 ng/ml) (A) or YM-254890 (100 nM) (C) or Batimastat and Ilomastat (E). The colony area of Raji was measured (15 colonies/condition) by using Leica Qwin image analysis software. B, D, F The same number of colonies (15 colonies/condition) was aseptically harvested from 96-well plates and stained with propidium iodide (PI) to detect PI-viable cells by flow cytometry. Absolute cell counts were obtained by the counting function of the MACSQuant® Analyzer. Bars represent the means ± SD of three independent experiments. The statistical significance between control and treated cultures was calculated using one-way ANOVA and the Bonferroni’s post-test was used to compare data. NT , not treated. *P < 0.05; **P < 0.01; ***P < 0.001.