Table 1.

Different molecules used in B-cell colony assays.

Reagent	Abbreviation	Final concentration	Role	Supplier
Epidermal growth factor	EGF	100 ng/ml	–	R&D Systems, Minneapolis, MN, USA
Sphingosine-1-phosphate	S1P	100 nM	–	Sigma-Aldrich, St. Louis, MO, USA
Endothelin-1	ET-1	100 nM	–	Sigma-Aldrich, St. Louis, MO, USA
Thrombin	Thr	2 U/ml	–	GE Healthcare, Chicago, IL, USA
AG1478	–	250 nM	EGFR inhibitor	Selleckchem, Houston, TX, USA
AG879	–	2 μM	ErbB2 inhibitor	Selleckchem, Houston, TX, USA
Wortmannin	WT	100 nM	PI3K inhibitor	Enzo, Farmingdale, NY, USA
Akt inhibitor VIII	Akt VIII i	1 μM	Akt inhibitor VIII	Sigma-Aldrich, St. Louis, MO, USA
PD98059	–	10 μM	Mitogen-activated protein kinase (MEK)/ERK1/2 inhibitor	Merck Life Science, Milano, Italy
YM-254890	–	100 nM	Gαq inhibitor	Adipogen Life Sciences, San Diego, CA, USA
Pertussis toxin	PTX	10 ng/ml	Gi inhibitor	Tocris Bioscience, Bristol, UK
Batimastat	–	20 nM	Metalloprotease (MMP) inhibitor	Tocris Bioscience, Bristol, UK
Ilomastat	–	1 μM	Metalloprotease (MMP) inhibitor	Tocris Bioscience, Bristol, UK
W146	–	100 nM	Selective antagonist of S1PR1	Sigma-Aldrich, St. Louis, MO, USA
JTE-013	–	10 nM	Selective antagonist of S1PR2	Sigma-Aldrich, St. Louis, MO, USA
TY-52156	–	100 nM	Selective antagonist of S1PR3	Sigma-Aldrich, St. Louis, MO, USA
CYM-50358	–	1 nM	Selective antagonist of S1PR4	Sigma-Aldrich, St. Louis, MO, USA
BQ123	–	0.65 μg/ml	Endothelin A receptor antagonist	Sigma-Aldrich, St. Louis, MO, USA
BQ788	–	0.65 μg/ml	Endothelin B receptor antagonist	Sigma-Aldrich, St. Louis, MO, USA
SCH79797	–	10 nM	Selective antagonist of PAR-1	Tocris Bioscience, Bristol, UK
tcy-NH2	–	10 μM	Selective antagonist of PAR-4	Tocris Bioscience, Bristol, UK
Monoclonal antibody to PAR1 (ATAP2, sc-13503)	mAb to PAR1	1 mg/ml	–	Santa Cruz Biotechnology, Dallas, TX, USA