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. 2021 Jun 14;4:736. doi: 10.1038/s42003-021-02272-1

Fig. 1. Characterization of SH-SHY5Y cell lines expressing Cas9.

Fig. 1

a Schematic representation of primary genome-wide screen performed in SH-SY5Y cells expressing Cas9. b Immunocytochemistry of endogenous tau (SP70), Cas9 and FLAG proteins in parental and stable cell lines expressing Cas9. Pooled cells (PC1) and single clones (SC2) are depicted. Scale bar represents 50 µm. c Tau (SP70) and Cas9 western blot analysis on SH-SH5Y/Cas9 (PC1) cells infected with control or MAPT gRNAs for 14, 21, or 30 days of editing. d Immunocytochemistry of endogenous tau in SH-SH5Y/Cas9 (PC1) cells infected with control or MAPT gRNAs for 7 days of editing. Scale bars represent 200 µm. Data is represented as mean fluorescence intensity ± SEM. Control is a non-target gRNA.