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. 2021 Jun 14;11:12468. doi: 10.1038/s41598-021-92015-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Histological image of an H&E stained brain section. (B) Representative FTIRI of the rat brain tissue shown in (A) with a rectangular label in hippocampal area indicating anatomic site examined by FTIRI. (C) Typical FTIR image from the rectangular labeled area before spectral processing. (D) The average spectra of hippocampal tissue of the control group in 4000–800 cm⁻¹ region (The spectra were normalized with respect to the Amide A band). (E) The pixel histogram for the image shown in (C).