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. 2021 Jun 14;11:12468. doi: 10.1038/s41598-021-92015-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Typical FTIR images of total saturated lipid content (A), carbonyl/lipid (B), unsaturated/saturated lipid (C), lipid acyl chain length (D), total protein content (E), lipid/protein (F), amide I/amide II (G) and glycogen/phosphate (H) band area ratios in left hippocampal tissue of control, 24 h and 1-month TBI groups. Color bars represent the scales for each of the parameters. Axes are in pixels, where one pixel is 6.5 μm. The pixel histograms for these images are shown in Fig. 3.