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. 2021 Jun 14;11:12428. doi: 10.1038/s41598-021-91985-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Protein expressions analyzed through SDS-PAGE and confirmed by Western Blot analysis. (A,B) SDS-PAGE of induced crude (1 mM-IPTG) proteins of CEMB-Cry1Ac, CEMB-Cry2A and CEMB-GTGene genes. (C) Purified proteins for Western Blot analysis. (D) Western Blot Analysis for CEMB-Cry1Ac protein, Lanes 1–3: CEMB-Cry1Ac protein (68 kDa), Lane M. Pre-strained protein ladder (Fermentas). (E) Western Blot Analysis for CEMB-Cry2Ac protein, Lanes 1–2: CEMB-Cry1Ac protein (71 kDa), Lane M: Pre-strained protein ladder (Fermentas). (F) Western Blot Analysis for CEMB-GTG protein, Lanes 1–4 CEMB-GTGene protein (54 kDa), Lane M. Pre-strained protein ladder (Fermentas).