Table 10.
Level of pest control and dose assessment of CEMB-Cry1Ac, CEMB Cry2A (µg/g) and CEMB-GTGene (µg/g) from five transgenic sugarcane lines in V2-generation.
| Serial no | Level of pest control and dose assessment at the plant age of 80 days | Glyphosate spray assay (3000 mL/hectare) | ELISA CEMB-GTGene dose assessment (µg/g) |
||
|---|---|---|---|---|---|
| Transgenic lines of sugarcane CPF-246 | Mortality (%age) | ELISA | |||
| CEMB-Cry1Ac (µg/g) | CEMB-Cry2A (µg/g) | ||||
| 1-CPF-246 (4L/2-9) 5 | 100% | 0.678 | 0.656 | Tolerant | 0.562 |
| 2-CPF-246 (5L/5-8) 4 | 100% | 0.678 | 0.601 | Tolerant | 0.572 |
| 3-CPF-246 (5L/5-8) 8 | 100% | 0.673 | 0.598 | Tolerant | 0.572 |
| 4-CPF-246 (6L/5-4) 2 | 100% | 0.680 | 0.598 | Tolerant | 0.590 |
| 5-CPF-246 (6L/5-4) 4 | 100% | 0.680 | 0.601 | Tolerant | 0.591 |
| 6-CPF-246 (6L/5-4) 5 | 100% | 0.682 | 0.591 | Tolerant | 0.595 |
| 7-CPF-246 (6L/5-4) 7 | 100% | 0.680 | 0.599 | Tolerant | 0.591 |
| 8-CPF-246 (6L/5-4) 8 | 100% | 0.677 | 0.596 | Tolerant | 0.594 |
| 9-CPF-246 (L8/4-3) 3 | 100% | 0.641 | 0.642 | Tolerant | 0.534 |
| 10-CPF-246 (L8/4-3) 9 | 100% | 0.635 | 0.640 | Tolerant | 0.540 |
| 11-CPF-246 (L8/4-3) 10 | 100% | 0.641 | 0.646 | Tolerant | 0.550 |
| 12-CPF-246 (L9/6-10) 2 | 100% | 0.646 | 0.611 | Tolerant | 0.561 |
| 13-CPF-246 (L9/6-10) 4 | 100% | 0.650 | 0.599 | Tolerant | 0.555 |
| 14-CPF-246 (L9/6-10) 8 | 100% | 0.639 | 0.596 | Tolerant | 0.567 |
| 15-CPF-246 (L9/6-10) 10 | 100% | 0.640 | 0.615 | Tolerant | 0.562 |