Table 2.

Biolistic transformation in sugarcane and selection of transgenic plants.

Variety	Total no of calli bombarded	No of calli survived on kanamycine selection (%)	No of calli survived on kanamycine + glyphosate selection (%)	No of calli regenerated on selection medium (%)	Regenerated plantlets on selection media	Plantlets after GUS assay screening multiplied in the tubes	PCR (GTGene) screening and in the process of acclimatization	In tubes on rooting media	Shifted to green house in soil pots	Survived (one and half month old plants) after spray 900 mL/ 80 L/acre	In field	2-month old plants after glyphosate spray 1100 mL/80 L	Spray tolerant (1200 mL/80 L/acr) plants further analyzed for three genes through PCR, Southern Blot, DIPSTICK and ELISA	Positive for CEMB-Cry1Ac	Positive for CEMB-Cry2A	Positive for CEMB-GTGene	Three genes positive plants (transgenic plants survived in field after glyphosate spray (1200 mL/80 L/acre)	Transformation efficiency (%)
SPF-213	100	70	34	21	500	1000	800	752	656	200	122	54	27	14	11	27	11	1.1
SPF-234	100	74	40	32	600	1000	700	680	525	250	141	90	39	15	13	39	13	1.3
HSF-240	100	45	29	13	300	1000	320	205	125	75	60	40	15	11	10	15	10	1
CPF-246	100	91	81	48	950	1000	966	900	900	900	751	300	66	30	15	66	15	1.5
Total													147	71	58	147	49