Table 5.

Molecular analysis of CPF-246 (L4/2-9) transgenic sugarcane plants from V₂-generation.

Serial no	PCR			Southern Blot analysis			Dipstick assay			ELISA (µg/g)			Glyphosate spray assay (1200 m/acre)
Transgenic lines of sugarcane CPF-246	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene
CPF-246 (4L/2-9) 1	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.675	0.645	0.565	Tolerant
CPF-246 (4L/2-9) 2	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.677	0.641	0.561	Tolerant
CPF-246 (4L/2-9) 3	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.598	0.596	0.551	Tolerant
CPF-246 (4L/2-9) 4	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.645	0.644	0.560	Tolerant
CPF-246 (4L/2-9) 5	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.678	0.656	0.562	Tolerant
CPF-246 (4L/2-9) 6	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.670	0.651	0.567	Tolerant
CPF-246 (4L/2-9) 7	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.671	0.655	0.570	Tolerant
CPF-246 (4L/2-9) 8	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.612	0.634	0.559	Tolerant
CPF-246 (4L/2-9) 9	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.615	0.629	0.545	Tolerant
CPF-246 (4L/2-9) 10	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.679	0.642	0.568	Tolerant