Table 6.

Molecular analysis of CPF-246 (L5/5-8) transgenic sugarcane plants from V₂-generation.

Serial no	PCR			Southern Blot analysis			Dipstick asaay			ELISA (µg/g)			Glyphosate spray assay (1200 m/acre)
Transgenic lines of sugarcane CPF-246	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene
CPF-246 (5L/5-8) 1	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.680	0.598	0.577	Tolerant
CPF-246 (5L/5-8) 2	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.670	0.590	0.560	Tolerant
CPF-246 (5L/5-8) 3	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.676	0.595	0.575	Tolerant
CPF-246 (5L/5-8) 4	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.678	0.601	0.572	Tolerant
CPF-246 (5L/5-8) 5	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.674	0.596	0.580	Tolerant
CPF-246 (5L/5-8) 6	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.670	0.590	0.578	Tolerant
CPF-246 (5L/5-8) 7	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.665	0.580	0.581	Tolerant
CPF-246 (5L/5-8) 8	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.673	0.598	0.572	Tolerant
CPF-246 (5L/5-8) 9	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.675	0.591	0.570	Tolerant
CPF-246 (5L/5-8) 10	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.678	0.588	0.575	Tolerant