Table 7.
Molecular analysis of CPF-246 (L6/5-4) transgenic sugarcane plants from V2-generation.
| Serial no | PCR | Southern Blot analysis | Dipstick assay | ELISA (µg/g) | Glyphosate spray assay (1200 m/acre) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Transgenic lines of sugarcane CPF-246 | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | CEMB-Cry1Ac | CEMB-Cry2A | CEMB-GTGene | |
| CPF-246 (6L/5-4) 1 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.685 | 0.605 | 0.589 | Tolerant |
| CPF-246 (6L/5-4) 2 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.680 | 0.598 | 0.590 | Tolerant |
| CPF-246 (6L/5-4) 3 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.681 | 0.596 | 0.585 | Tolerant |
| CPF-246 (6L/5-4) 4 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.680 | 0.601 | 0.591 | Tolerant |
| CPF-246 (6L/5-4) 5 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.682 | 0.591 | 0.595 | Tolerant |
| CPF-246 (6L/5-4) 6 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.679 | 0.595 | 0.586 | Tolerant |
| CPF-246 (6L/5-4) 7 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.680 | 0.599 | 0.591 | Tolerant |
| CPF-246 (6L/5-4) 8 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.677 | 0.596 | 0.594 | Tolerant |
| CPF-246 (6L/5-4) 9 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.621 | 0.610 | 0.588 | Tolerant |
| CPF-246 (6L/5-4) 10 | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | +VE | 0.678 | 0.611 | 0.585 | Tolerant |