Table 8.

Molecular analysis of CPF-246 (L8/4-3) transgenic sugarcane plants from V₂-generation.

Serial no	PCR			Southern Blot analysis			Dipstick assay			ELISA (µg/g)			Glyphosate spray assay (1200 ml/acre)
Transgenic lines of sugarcane CPF-246	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene
CPF-246 (L8/4-3) 1	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.645	0.640	0.551	Tolerant
CPF-246 (L8/4-3) 2	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.640	0.644	0.542	Tolerant
CPF-246 (L8/4-3) 3	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.641	0.642	0.534	Tolerant
CPF-246 (L8/4-3) 4	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.640	0.622	0.538	Tolerant
CPF-246 (L8/4-3) 5	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.630	0.635	0.525	Tolerant
CPF-246 (L8/4-3) 6	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.638	0.635	0.535	Tolerant
CPF-246 (L8/4-3) 7	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.642	0.632	0.552	Tolerant
CPF-246 (L8/4-3) 8	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.635	0.638	0.551	Tolerant
CPF-246 (L8/4-3) 9	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.635	0.640	0.540	Tolerant
CPF-246 (L8/4) 10	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.641	0.646	0.550	Tolerant