Table 9.

Molecular analysis of CPF-246 (L9/6-10) transgenic sugarcane plants from V₂-generation.

Serial no	PCR			SOUTHERN			DIPSTICK			ELISA (µg/g)			Glyphosate spray assay (1200 m/acre)
Transgenic lines of sugarcane CPF-246	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene	CEMB-Cry1Ac	CEMB-Cry2A	CEMB-GTGene
CPF-246 (L9/6-10) 1	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.648	0.620	0.565	Tolerant
CPF-246 (L9/6-10) 2	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.646	0.611	0.561	Tolerant
CPF-246 (L9/6-10) 3	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.644	0.603	0.558	Tolerant
CPF-246 (L9/6-10) 4	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.650	0.599	0.555	Tolerant
CPF-246 (L9/6-10) 5	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.640	0.598	0.560	Tolerant
CPF-246 (L9/6-10) 6	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.645	0.599	0.559	Tolerant
CPF-246 (L9/6-10) 7	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.641	0.610	0.556	Tolerant
CPF-246 (L9/6-10) 8	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.639	0.596	0.567	Tolerant
CPF-246 (L9/6-10) 9	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.633	0.613	0.560	Tolerant
CPF-246 (L9/6-10) 10	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	+VE	0.640	0.615	0.562	Tolerant