Table 3.
Leading terms of enriched Gene Ontology biological process (GO) with ClueGO classification program and differentially expressed jejununal mucosa proteins of heat stress vs pair-fed dairy cows.
| GO:ID | GO term | Number of proteins involved | Protein names | P value |
|---|---|---|---|---|
| GO:0000302 | Response to reactive oxygen species | 3 | CAT, RPS3, SOD2 | 4.54E−02 |
| GO:0043462 | Regulation of ATPase activity | 3 | ACTN1, ALDOB, HSPA1A | 1.06E−02 |
| GO:0050821 | Protein stabilization | 3 | CALR, GAPDH, HSP90AA1 | 3.32E−02 |
| GO:0007006 | Mitochondrial membrane organization | 3 | HSP90AA1, HSPA4, SLC25A6 | 2.00E−02 |
| GO:0019210 | Kinase inhibitor activity | 3 | HSPB1, NPM1, QARS | 1.81E−02 |
| GO:1901224 | Positive regulation of NIK/NF-kappaB signaling | 3 | ACTN4, CALR, RPS3 | 1.98E−02 |
| GO:0009408 | Response to heat | 3 | HSP90AA1, HSPA1A, VCP | 4.14E−04 |
| GO:0007041 | Lysosomal transport | 3 | HSPA1A, HSPA8, VCP | 4.14E−04 |
| GO:0035967 | Cellular response to topologically incorrect protein | 5 | CALR, CANX, HSPA1A, HSPA8, VCP | 4.14E−04 |
| GO:0071826 | Ribonucleoprotein complex subunit organization | 5 | HSP90AA1, PRPF8, RPS19, SNRNP200, VCP | 4.14E−04 |
| GO:0016903 | Oxidoreductase activity, acting on the aldehyde or oxo group of donors | 3 | ALDH18A1, DLD, GAPDH | 4.87E−05 |
| GO:0072524 | Pyridine-containing compound metabolic process | 5 | ALDOB, GAPDH, NNT, TALDO1, VCP | 4.87E−05 |
| GO:0045333 | Cellular respiration | 3 | CAT, DLD, VCP | 4.87E−05 |
| GO:0016620 | Oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor | 3 | ALDH18A1, DLD, GAPDH | 4.87E−05 |
| GO:0009141 | Nucleoside triphosphate metabolic process | 6 | ADA, ALDOB, ATP5F1, DLD, GAPDH, VCP | 4.87E−05 |
| GO:0019362 | Pyridine nucleotide metabolic process | 5 | ALDOB, GAPDH, NNT, TALDO1, VCP | 4.87E−05 |
| GO:0006090 | Pyruvate metabolic process | 3 | ALDOB, DLD, GAPDH | 4.87E−05 |
| GO:0009124 | Nucleoside monophosphate biosynthetic process | 3 | ADA, ATP5F1, VCP | 4.87E−05 |
| GO:0009199 | Ribonucleoside triphosphate metabolic process | 5 | ALDOB, ATP5F1, DLD, GAPDH, VCP | 4.87E−05 |
| GO:0046496 | Nicotinamide nucleotide metabolic process | 5 | ALDOB, GAPDH, NNT, TALDO1, VCP | 4.87E−05 |
| GO:0009156 | Ribonucleoside monophosphate biosynthetic process | 3 | ADA, ATP5F1, VCP | 4.87E−05 |
| GO:0009167 | Purine ribonucleoside monophosphate metabolic process | 6 | ADA, ALDOB, ATP5F1, DLD, GAPDH, VCP | 4.87E−05 |
| GO:0009205 | Purine ribonucleoside triphosphate metabolic process | 5 | ALDOB, ATP5F1, DLD, GAPDH, VCP | 4.87E−05 |
| GO:0009168 | Purine ribonucleoside monophosphate biosynthetic process | 3 | ADA, ATP5F1, VCP | 4.87E−05 |
P value was calculated according to a hypergeometric test and corrected with Bonferroni stepdown in the ClueGo software.