Fig. 1. The expression of ACTBP2 in ECs pre-incubated with Aβ1–42 and the effect on the BBB permeability in Aβ1–42 microenvironment.
A Relative expression of COX5BP6, YWHABP1, and ACTBP2 in Aβ1–42-incubated ECs by qRT-PCR. Data are presented as mean ± SD (n = 3), *P < 0.05 versus ECs group, **P < 0.01 versus ECs group. B Fluorescence in situ hybridization (FISH) analysis of the location of ACTBP2 (green) mainly in the nucleus of Aβ1–42-incubated ECs. Scale bar represents 30 μm. C, D Effects of ACTBP2 on TEER values (C) and HRP flux (D) in Aβ1–42 microenvironment. E Effects of ACTBP2 on ZO-1, occludin, and claudin-5 expression levels in ECs pre-incubated with Aβ1–42 determined by qRT-PCR. F Effects of ACTBP2 on ZO-1, occludin, and claudin-5 expression levels in ECs pre-incubated with Aβ1–42 determined by western blot. Data are presented as mean ± SD (n = 3, each). **P < 0.01 versus shNC group. ##P < 0.01 versus shACTBP2+vector group. G Effects of ACTBP2 on ZO-1, occludin, and claudin-5 expression levels and distribution in Aβ1–42 microenvironment determined by immunofluorescence staining. ZO-1, occludin, and claudin-5 (green) were labeled with secondary antibodies against anti-ZO-1, anti-occludin, and anti-claudin-5 antibodies, respectively, and nuclei (blue) were labeled with DAPI. Scale bar represents 30 μm.