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. 2021 Jun 14;7:142. doi: 10.1038/s41420-021-00531-y

Fig. 5. KHDRBS2-regulated BBB permeability by increasing the half-life of HEY2 mRNA in Aβ1–42 microenvironment.

Fig. 5

A Effects of KHDRBS2 on HEY2 mRNA expression in ECs pre-incubated with Aβ1–42 by qRT-PCR. B Effects of KHDRBS2 on HEY2 protein expression in ECs pre-incubated with Aβ1–42 by western blot. Data are presented as mean ± SD (n = 3, each). **P < 0.01 versus shNC group. ##P < 0.01 versus shKHDRBS2+vector group. C RNA immunoprecipitation confirmed the bonding between KHDRBS2 and HEY2 mRNA in Aβ1–42 microenvironment. Relative enrichment was measured by qRT-PCR. Data are presented as mean ± SD (n = 3, each). **P < 0.01 versus Anti-IgG group. D Western blot of the proteins from HEY2 CDS, HEY2 3′UTR, and antisense HEY2 pull-down assays in Aβ1–42 microenvironment. E Nascent HEY2 levels in Aβ1–42-incubated ECs by qRT-PCR. Data are presented as mean ± SD (n = 3, each). F The graph shows HEY2 levels in ECs pre-incubated with Aβ1–42 at different times treated with ActD in the Control, shNC, shKHDRBS2, shKHDRBS2+vector, and shKHDRBS2 + KHDRBS2 OE groups by qRT-PCR. G, H Effects of KHDRBS2 knockdown and HEY2 on TEER values G and HRP flux H in Aβ1–42 microenvironment. I Effects of KHDRBS2 knockdown and HEY2 on ZO-1, occludin, and claudin-5 expression levels in Aβ1–42-incubated ECs by western blot. Data are presented as mean ± SD (n = 3, each). *P < 0.05 versus shKHDRBS2+shNC group, **P < 0.01 versus shKHDRBS2+shNC group, #P < 0.05 versus shKHDRBS2+ vector group, ##P < 0.01 versus shKHDRBS2+vector group.