Fig. 6. HEY2 decreased the Promoter Activity of ZO-1, occludin, and claudin-5 in ECs pre-incubated with Aβ1–42.
A–C Schematic depiction of the different reporter plasmids and relative luciferase activity in Aβ1–42 microenvironment. The relative luciferase activity was conducted after Aβ1–42-incubated ECs co-transfected with ZO-1 A, occludin B, or claudin-5 C promoter (−1000 to 0 bp) (or promoter-deleted putative HEY2-binding sites) with pEX3-HEY2 or pEX3 empty vector. Data represent mean ± SD (n = 3, each). **P < 0.01, *P < 0.05. D–F HEY2 interacted with the promoters of ZO-1 D, occludin E, and claudin-5 F in Aβ1–42-incubated ECs. The transcription start site (TSS) was designated as +1. Putative HEY2-binding sites are illustrated. Immunoprecipitated DNA was amplified by PCR. Normal rabbit IgG was used as a negative control.