Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 14;11:12454. doi: 10.1038/s41598-021-91870-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Example of FACS analysis of the PBMC proliferation assay using the FL-1 channel (a) PBMC proliferation assay using fMSC (i) control unstimulated fPBMC, (ii) control activated fPBMC, (iii) activated fPBMC + primedc fMSC donor 1, (iv) activated fPBMC + primed fMSC donor 2, (v) activated fPBMC + primed fMSC donor 3, (vi) activated fPBMC + primed fMSC donor 4 compared to (b) PBMC assay using mMSC (i) control unstimulated fPBMC, (ii) control activated fPBMC, (iii) activated fPBMC + primed mMSC donor 1, (iv) activated fPBMC + primed mMSC donor 2, (v) activated fPBMC + primed mMSC donor 3, (vi) activated fPBMC + primed mMSC donor 4. (c) Individual MSC mediated immune suppression of both male and female PBMC expressed as a percentage indicatiing the suppression rate and (d)suppression (mean ± SEM) of fPBMC (n = 3) and mPBMC (n = 3) against both fMSC (n = 4) and mMSC (n = 4).