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. 2021 Jun 14;11:12454. doi: 10.1038/s41598-021-91870-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of fMSC and fPBMC whilst blocking the activity of IDO1 with (EPA) (a) effects of EPA on suppression PBMC (% suppression), (b) EPA effects on MSC mediated PBMC mean fluorescence intensity (MFI) and (c) effect of MSC IDO1 secretion by adding EPA to a co-culture. (d) Mean IDO1 secretion (n = 8) can be correlated (R² = 0.77) to MSC mediated immune suppression (n = 8) and can be predicted by the equation (+) = added to culure, (−) = not added to culture.