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. 2021 Jun 14;11:12487. doi: 10.1038/s41598-021-91792-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gene Ontology enrichment profiles for the nitrosamines and DMF. Genes lacking in the sensitive strains were enriched for certain biological processes including; NDMA: arginine biosynthesis, fatty acid metabolic process, mitochondrial genome maintenance and macroautophagy. NDEA: protein transport, vacuolar transport, arginine biosynthesis, negative regulation of translation and macroautophagy. NMBA: protein transport, protein targeting to vacuole and cell morphogenesis. DMF: arginine biosynthesis, protein transport and iron–sulfur cluster assembly. Each node represents a gene set while the node size represents the number of genes in the gene set. The edge represents overlap between gene sets while the edge width represents the number of genes that overlap between connected gene sets. Not all pathways enriched at significant FD score are shown, e.g. septin ring assembly for NDEA, etc. Enrichment profiles were generated using the Bioconductor v3.12, https://www.bioconductor.org/.