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. 2021 Jun 14;11:12497. doi: 10.1038/s41598-021-91507-6

Figure 2.

Figure 2

Effect of selumetinib on acrolein-induced oxidative stress. (A) Primary cultured cortical neurons were treated with acrolein (0–30 μM). (B,C) Primary cultured cortical neurons were treated with acrolein (30 μM) with/without selumetinib (SEL, 10 μM) for 24 h. Western blot assay was employed to measure HO-1 (A), Nrf-2 (B) and HO-1 (C). Each lane contained 30 μg protein for all experiments. Graphs show statistical results from relative optical density of bands on the blots. Values are the mean ± SEM (n = 3/treatment). *p < 0.05 statistically significant in the acrolein groups compared with the control groups; #p < 0.05 statistically significant in acrolein plus SEL group compared with acrolein group by one-way analysis of variance (one-way ANOVA) and followed by the LSD test as post-hoc method.