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. 2021 Jun 14;11:12427. doi: 10.1038/s41598-021-91684-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Peptide to protein mapping: (A) Circos plots describing the mapping of peptides to specific chromosomes through parent protein. The right-hand side details the information of protein count heat map and enrichment of in three stages. (B) Pearson correlation-based similarity matrix plot of all chromosomes and associated target proteins determined with respect to endogenous peptides. (C) Heat map presentation of proteins quantitative data for common parental proteins among three stages. Fold change is calculated using the heifer as the control for lactation and pregnancy. Proteins were grouped based on the k-mean clustering and fold change calculate with respective color coding.