Table 1.

# Total number of unassigned spectra acquired in all the raw data mapped in COMET search engine using no enzyme search parameter.

Total raw files	Conditions	Unassigned total spectra#	Sum total spectra (after assignment)	Endogenous De novo Peptides	Proteins (Protein prophet probability=1) (Iprophet probability$\ge$0.999999)
20	Heifer	214471	27083	5239	2092 (900)*
20	Pregnant	218556	27336	4774	2002 (755)*
20	Lactation	212208	27025	5466	1946 (860)*

*Proteins reported with bold letters asterisk symbol are identified with more than 10 percent sequence coverage peptides of respective protein and all the peptides identified through minimum of 2 independent spectra.