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. 2021 Jun 14;12:3604. doi: 10.1038/s41467-021-23879-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Selection signatures in the comparisons between ECs and WCs. Possible genes hypothetically under selection within the putative selective regions are labeled in red. b Significant divergence of the malate content between ECs (n = 222) and WCs (n = 133). In the violin plots (significance was tested with Wilcoxon tests), central line: median values; bounds of box: 25th and 75th percentiles; whiskers: 1.5 * IQR (IQR: the interquartile range between the 25th and 75th percentile). c Relative expression levels of the five putative ALMT genes in fruits of three low-malate EC accessions vs. three high-malate WC accessions at 48 DAA (days after anthesis). The number (n) of individuals for each accession is shown below. Data were presented as the mean ± SE. Significance was tested with two-sided Student’s t-tests. d A significantly elevated level of malate content (left) in peach mesocarp tissues transiently over-expressing (TOE) PpALMT1 (right). Fruit mesocarps transiently transformed with the empty vector were used as controls (CK). The number (n) of each independent experiment is shown below. Data were presented as the mean ± SD. Significance was tested with two-sided Student’s t-tests. Source data underlying Fig. 3c, d are provided as a Source Data file.