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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Addict Biol. 2020 Dec 15;26(4):e12994. doi: 10.1111/adb.12994

Figure 2. Epigenetic modifications at dopamine related genes across developmental time points in the VTA.

Figure 2.

A.) Timeline of developmental time points. B.) Nr4a2 is activated at PND 84, (Students two-tailed ttest; PND 84, p=0.029, adjusted p = 0.012). C.) Cartpt is activated at PND 84 (Students two-tailed ttest; PND 84, p=0.013, adjusted p = 0.019). D.) Dat is repressed at PND 112 (Students two-tailed ttest; PND 112, p=0.015, adjusted p = 0.047). E.) No significant changes in Th. F.) H3K27me3 is depleted at the Nr4a2 promotor at PND 84 (Students two-tailed ttest; PND 84, p=0.012, adjusted p = 0.017). G.) H3K27me3 is depleted at the Cartpt promotor at PND 56 and 84 (Students two-tailed ttest; PND 56, p=0.001, adjusted p = 0.005; PND 84, p=0.002, adjusted p = 0.005). H.) H3K27me3 is enriched at the Dat promotor at PND 112 (Students two-tailed ttest; PND 112, p=0.006, adjusted p = 0.040). I.) H3K27me3 is depleted at the Th promotor at PND 56 (Students two-tailed ttest; p=0.029, adjusted p = 0.012). All data normalized to GAPDH relative to PND28. n = 5 – 12 mice per group. Error bars represent S.E.M. *p<.05 relative to PND28.